Journal: Neural Regeneration Research
Article Title: Dynamic expression of Slit1–3 and Robo1–2 in the mouse peripheral nervous system after injury
doi: 10.4103/1673-5374.268930
Figure Lengend Snippet: Fold-changes of Slit2, Slit3, and Robo1 mRNA in sciatic nerve, and Slit1–3 and Robo1–2 protein expression in sciatic nerve. (A) Fold-changes of Robo1 mRNA in sciatic nerve detected by quantitative real-time polymerase chain reaction. (B) Slit2, Slit3, and Robo1 mRNA fold-changes in the mouse distal sciatic nerve from data set GSE74087. (C) Slit2, Slit3, and Robo1 mRNA fold-changes in the mouse distal sciatic nerve from data set GSE22291. (D) Western blot results for Slit1–3 and Robo1–2 protein expression in transected mouse sciatic nerve. (E) Quantification of Slit1–3 and Robo1–2 protein levels in intact sciatic nerve, proximal nerve stump, nerve bridge, and distal nerve stump. Data are expressed as mean ± SEM ( n = 3). * P < 0.05, ** P < 0.01, vs . control group (one-way analysis of variance followed by Student’s t -test). B: Bridge, C: control, D: distal; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; P: proximal. Robo: Roundabout.
Article Snippet: Rabbit anti-Slit1 (#SAB1307048) and anti-Slit3 (#SAB2104337) antibodies were purchased from Sigma.
Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot